Terceiro Molar Ectópico em Incisura Mandibular / Ectopic Third Molar in the Mandibular Notch

Dentes ectópicos são frequentemente encontrados, entretanto, terceiros molares inferiores ectópicos são inco-muns, com etiologia obscura e pouco descritos na literatura pertinente. Sua localização já foi relatada nas regiões condilar, subcondilar, incisura mandibular, ângulo e borda inferior da mandíbula. Devido à importância do plane-jamento terapêutico, manejo adequado e variedade das manifestações clínicas desta condição, este trabalho teve como objetivo relatar um caso clínico de terceiro molar inferior ectópico na região de incisura mandibular, com radiolucência aumentada ao redor da coroa, em um paciente do gênero masculino, de 28 anos e sem sintomato-logia. Acompanhamento radiográfico pode ser indicado, no entanto, em pacientes sintomáticos ou com alterações patológicas associadas, a extração deve ser considerada. Diante disso, o tratamento de escolha foi a extração do dente 38 sob anestesia geral, por via intraoral, tendo em vista a posição dentária, radiolucência e morbidade as-sociada à cirurgia. O tecido mole circundante foi enviado para análise anatomopatológica. O paciente evoluiu sem intercorrências durante avaliação pós-operatória (AU)

Ectopic teeth are frequently found, however, ectopic lower third molars are uncommon, with obscure etiology and little described in the pertinent literature. Its location has been reported in the condylar, subcondylar, mandibular notch, angle and lower edge of the mandible. Due to the importance of therapeutic planning, adequate manage-ment and variety of clinical manifestations of this condition, this study aimed to report a clinical case of an ectopic lower third molar in the region of the mandibular notch, with increased radiolucency around the crown, in a male gender pacient, 28 years old and without symptoms. Radiographic follow-up may be indicated, however, in symp-tomatic patients or patients with associated pathological changes, extraction should be considered. Therefore, the treatment of choice was the extraction of tooth 38 under general anesthesia, intraorally approach, considering the dental position, radiolucency and morbidity associated with the surgery. The surrounding soft tissue was sent for anatomopathological analysis. The patient evolved uneventfully during the postoperative evaluation (AU)

Automatic Segmentation of Bone Canals in Histological Images.

The literature provides many works that focused on cell nuclei segmentation in histological images. However, automatic segmentation of bone canals is still a less explored field. In this sense, this paper presents a method for automatic segmentation approach to assist specialists in the analysis of the bone vascular network. We evaluated the method on an image set through sensitivity, specificity and accuracy metrics and the Dice coefficient. We compared the results with other automatic segmentation methods (neighborhood valley emphasis (NVE), valley emphasis (VE) and Otsu). Results show that our approach is proved to be more efficient than comparable methods and a feasible alternative to analyze the bone vascular network.

Urgent surgical approach for comminuted mandible fracture and considerations regarding airway risk. Case report / Abordagem cirúrgica urgente para fratura cominutiva da mandíbula e considerações sobre o risco das vias aéreas. Relato de caso

Introduction: Jaw fractures are one of the most common sites of maxillofacial injuries. The location of the jaw makes it very vulnerable to direct impacts. The purpose of treatment is to restore aesthetic function. Case Report: The purpose of this report is to present a case of comminuted mandible fracture in a young male patient, treated urgently due to the need for maintenance of the airways, where fracture osteosynthesis surgery was submitted. Conclusion: The initial assessment of these trauma patients should follow the Trauma Life Support protocol, and structural damage should be investigated. Introduction: Jaw fractures are one of the most common sites of maxillofacial injuries. The location of the jaw makes it very vulnerable to direct impacts. The purpose of treatment is to restore aesthetic function. Case Report: The purpose of this report is to present a case of comminuted mandible fracture in a young male patient, treated urgently due to the need for maintenance of the airways, where fracture osteosynthesis surgery was submitted. Conclusion: The initial assessment of these trauma patients should follow the Trauma Life Support protocol, and structural damage should be investigated

Introdução: As fraturas de mandíbula são um dos locais mais comuns de lesões maxilofaciais. A localização da mandíbula a torna muito vulnerável a impactos diretos. O objetivo do tratamento é restaurar a função estética. Relato de Caso: O objetivo deste relato é apresentar um caso de fratura cominutiva de mandíbula em paciente jovem do sexo masculino, atendido com urgência devido à necessidade de manutenção das vias aéreas, onde foi submetido cirurgia de osteossíntese da fratura. Conclusão: A avaliação inicial desses pacientes com trauma deve seguir o protocolo do Suporte de Vida no Trauma, e devem ser investigados danos estruturais.

Important aspects during advanced lingual laceration management: report of 2 cases / Aspectos importantes para o manejo das lacerações complexas da língua: relato de 2 casos

ABSTRACT Intraoral injuries affect the stomatognathic system, creating difficulties in mastication and speech articulation, especially when they affect the tongue. In this context, the quality of the suture and local care are important to proper recovery and the patient's brief return to their normal functions. Oral lacerations resulting from trauma require specific approaches. Treatments of this type of trauma may primarily be performed by professionals who conduct emergency care; however, they may require the attention of specialists. This article contains recommendations for the primary approach, treatment, and postoperative care of complex lacerations in the tongue.

RESUMO As lesões intraorais afetam o sistema estomatognático, gerando dificuldades na mastigação e na articulação da fala, principalmente quando afetam a língua. Nesse contexto, a qualidade da sutura e o cuidado local são importantes para a recuperação adequada e o breve retorno do paciente às suas funções normais. As lacerações orais resultantes de trauma requerem abordagens específicas. Os tratamentos desse tipo de trauma podem ser realizados principalmente por profissionais que realizam atendimento de emergência; no entanto, eles podem exigir a atenção de especialistas. Este artigo contém recomendações para a abordagem primária, tratamento e cuidados pós-operatórios de lacerações complexas da língua.

Infecção cervicofacial grave de origem odontogênica: relato de caso / Severe cervicofacial infection of odontogenic origin: case report

Introdução: as infecções odontogênicas tem como principal origem a necrose pulpar com invasão bacteriana no tecido periapical e periodontal, podendo levar à formação de quadros de celulite e posteriormente de abscesso, além disso, possuem o potencial de disseminar-se pelos espaços faciais profundos e comprometer a vida do paciente. Complicações graves, decorrentes dos quadros de infecções odontogênicas, podem ocorrer, se o tratamento instituído não for adequado, como: trombose do seio cavernoso, abscesso cerebral, mediastinite e até óbito. Objetivo: discutir o manejo das infecções odontogênicas disseminados em espaços faciais profundos, através do relato de caso clínico. Caso clínico: paciente de 52 anos, portador de diabetes mellitus tipo 2, com infecção odontogênica, no exame clínico inicial apresentava trismo, disfonia, dispneia, disfagia, hiperemia e edema em lado esquerdo da face, envolvendo os espaços canino, bucal, submandibular e cervical além de unidades dentárias com foco infeccioso. Em exame de tomográfica computadorizada, observou-se desvio da via área, presença de um grande volume de gás e de lojas de infecção. Optou-se como tratamento a remoção dos focos dentários e drenagem intraoral foi realizada pela equipe da CTBMF com anestesia local e posteriormente drenagem sob anestesia geral, intubação com uso de fibroscopia pela equipe de cirurgia cabeça e pescoço. Considerações finais: as infecções odontogênicas que envolvem espaços faciais profundos devem ser tratadas com urgência e o tratamento de escolha dessa condição deve ser remoção imediata do foco infeccioso, exploração e drenagem rápida e agressiva dos espaços faciais envolvidos e associação com antimicrobianos de amplo espectro com características bactericidas.(AU)

Introduction: the main origin of odontogenic infections is pulp necrosis with bacterial invasion in the periapical and periodontal tissue, which may lead to the formation of cellulitis and later abscess, besides having the potential to spread to the deep facial spaces and compromise the patient's life. Severe complications from dental infections may occur if the treatment is not appropriate, such as cavernous sinus thrombosis, brain abscess, mediastinitis and even death. Objective: to discuss the management of disseminated odontogenic infections in deep facial spaces through a case report. Case report: a 52-year-old patient with type 2 diabetes mellitus, with odontogenic infection, presented at the initial clinical examination trismus, dysphonia, dysphagia, dysphagia, hyperemia and edema on the left side of the face involving the canine, buccal, submandibular and cervical spaces. of dental units with infectious focus. CT scan revealed deviation of the airway, presence of a large volume of gas and infection stores. The treatment was chosen to remove dental foci and intraoral drainage was performed by the CTBMF team under local anesthesia and subsequently under general anesthesia drainage, intubation with fibroscopy by the head and neck surgery team. Final considerations: odontogenic infections involving deep facial spaces should be treated urgently and the treatment of choice for this condition should be immediate removal of the infectious focus, rapid and aggressive exploration and drainage of the involved facial spaces and association with broad-spectrum antimicrobials with bactericidal characteristics.(AU)

Reconstrução de fratura panfacial cominuta ­ relato de caso / Reconstruction of panfacial fracture - case report

RESUMO: Modelo de estudo: Relato de Caso. Importância do problema: As fraturas panfaciais recebem essa denominação quando os terços faciais apresentam fraturas concomitantes. Com frequência, essas lesões estão relacionadas a danos importantes aos tecidos moles, cominuição e perda de segmentos ósseos e/ou dentários, que podem gerar má oclusão e graves deformidades faciais, visto que etiologia de tal condição se deve à acidentes de alta dissipação de energia. O tratamento das fraturas panfaciais com o uso de fixação interna rígida permite restaurar as funções mastigatórias, bem como os contornos faciais. Comentários: O objetivo desse trabalho é relatar a reconstrução de uma fratura panfacial, envolvendo a mandíbula e com elevado grau de cominuição do complexo zigomático orbitário e do arco zigomático, em uma paciente do sexo feminino, em que acesso hemicoronal e retromandibular foram escolhidos para reconstrução e reestruturação do complexo facial. Conclusão: O correto manejo das fraturas panfaciais, é um dos grandes desafios do cirurgião buco maxilo facial, visto o nível de dificuldade para reestabelecer de maneira satisfatória as condições estéticas e funcionais existentes previamente ao trauma. Diferentes sequências de tratamento vêm sendo propostas, podendo ser utilizadas com sucesso após análise adequada do caso clínico e correta indicação. (AU)

ABSTRACT: Study model: Case Report. Importance of the problem: Panfacial fractures receive this designation when the facial thirds have concomitant fractures. Frequently, these lesions are related to important soft tissue damage, comminution, and loss of bone and/or dental segments, which may lead to malocclusion and severe facial deformities, since the etiology of such condition is due to accidents of high energy dissipation. The treatment of the panfacial fractures with the use of rigid internal fixation allows restoring the masticatory functions, as well as the facial contours. Comments: This study aimed to report the reconstruction of a panfacial fracture, involving the mandible and with a high degree of comminution of the zygomatic or zygomatic arch, in a female patient, whose hemicoronal and retromandibular access were chosen for reconstruction and restructuring of the facial complex. Conclusion: The correct management of panfacial fractures is one of the greatest challenges of the maxillofacial surgeon, given the level of difficulty to satisfactorily reestablish the aesthetic and functional conditions existing before the trauma. Different treatment sequences have been proposed to be used successfully after adequate analysis of the clinical case and correct indication. (AU)

Removal of firearm projectile from the maxillary sinus: a case report / Remoção de projétil de arma de fogo do seio maxilar: relato de caso

ABSTRACT Foreign bodies in the paranasal sinuses after critical accidents are rare occurrences that have sporadically been reported worldwide. Firearm projectiles, daggers, knives, glass fragments, stones, teeth, and wood can get lodged in the soft and hard tissues of the face. The management and prognosis in these cases depend on the composition and location of the foreign body as well as on the presence or absence of infection. Although there are reports involving the soft tissues of the face, the ethmoid, sphenoid, frontal, and maxillary sinuses are the most frequently involved sites. The main manifestation of this condition is sinusitis arising from infection and/or inflammation caused by the foreign body, which is a contaminated object. We report a case of removal of a firearm projectile located in the maxillary sinus of a 34-year-old patient under local anesthesia.

RESUMO Corpos estranhos nos seios paranasais são ocorrências raras que têm sido esporadicamente relatadas na literatura em todo o mundo. Projéteis de arma de fogo, punhais, facas, fragmentos de vidro, pedras, dentes e madeira podem ficar alojados nos tecidos moles e duros do rosto. O manejo e o prognóstico nesses casos dependem da composição e localização do corpo estranho, bem como da presença ou ausência de infecção. Embora haja relatos envolvendo os tecidos moles da face, os seios etmoidal, esfenoidal, frontal e maxilar são os locais mais frequentemente envolvidos. A principal manifestação dessa condição é sinusite decorrente de infecção e / ou inflamação causada pelo corpo estranho, que é um objeto contaminado. Relatamos um caso de remoção de um projétil de arma de fogo localizado no seio maxilar de um paciente de 34 anos de idade, sob anestesia local.

Um raro diagnóstico de osteoma periférico em palato duro: relato de caso / A rare diagnosis of peripheral osteoma in the hard palate: case report

Objetivo: relatar uma biópsia excisional de um osteoma periférico no palato duro direito de uma paciente jovem, do sexo feminino e não sindrômica. Relato de caso: paciente do sexo feminino, 32 anos de idade, melanoderma e normossitêmico, buscou atendimento no ambulatório de diagnóstico estomatológico da Faculdade de Odontologia da Universidade Federal de Uberlândia com queixa quanto ao surgimento de lesão na região de palato. Após avaliação clínica e imaginológica, foi decidido realizar biópsia excisional sob anestesia local da lesão. Foram levantadas três hipóteses de diagnóstico: osteoma periférico, exostose ou osteossarcoma. O material coletado foi enviado para análise histopatológica, que confirmou a hipótese de osteoma periférico. Considerações finais: os osteomas periféricos na região de palato duro são raríssimos. Essa lesão geralmente é assintomática ­ exceto quando há impacto traumático durante a mastigação ­, de crescimento lento, podendo se desenvolver em osso cortical ou medular. O tratamento de escolha é a remoção cirúrgica e o índice de recorrência é raro. O caso enfatiza a condição rara, pois foram encontrados apenas seis casos semelhantes relacionados na literatura. (AU)

Objective: To report an excisional biopsy of a peripheral osteoma in the right hard palate in a non-syndromic young female patient. Case Report: A 32-year-old female patient, black, and normosystemic sought assistance in the stomatological diagnosis outpatient clinic of the School of Dentistry of the Federal University of Uberlândia, Brazil, complaining about the appearance of a lesion in the palate region. After clinical and imaging assessment, it was decided to perform an excisional biopsy under local anesthesia of the lesion. Three diagnostic hypotheses were raised - peripheral osteoma, exostosis, or osteosarcoma. The material collected was sent for histopathological analysis, which confirmed the hypothesis of peripheral osteoma. Final considerations: Peripheral osteomas in the hard palate region are very rare. This lesion is usually asymptomatic, except when there is a slow-growing chewing impact during mastication, which may develop in cortical or spinal bone. Surgical removal is the treatment of choice and the rate of recurrence is rare. The case emphasizes the rare condition, considering only six similar cases were found in the literature. (AU)

Tratamento cirúrgico de terceiro molar inferior invertido: relato de caso / Treatment of third invested lower molar: case report

A presença de terceiros molares invertidos se apresenta como uma condição pouco relatada na literatura. Diversas hipóteses têm sido propostas para explicar o deslocamento destes elementos, entretanto suas causas e tratamentos permanecem incertos. O objetivo deste trabalho é relatar um caso clínico de impacção invertida de terceiro molar inferior direito, onde optou-se pela exodontia sob anestesia local com osteotomia conservadora e exérese total do elemento em paciente do sexo feminino de 52 anos. Terceiros molares com esta variação de erupção são raros na clínica, sendo geralmente visualizados em exames radiográficos de rotina e observados clinicamente após início de sintomatologia. Diversos tratamentos têm sido propostos, variando de abordagem cirúrgica, ao acompanhamento de longo prazo. A abordagem intraoral associada a uma conduta conservadora tem se mostrado o tratamento de escolha na maioria dos casos. Entretanto, a escolha do planejamento dependerá, dentre outros fatores, da sintomatologia do paciente e a presença de patologias associadas, sempre optando-se pela técnica que promoverá menor morbidade operatória e melhor resolubilidade do caso(AU)

The presence of inverted third molars presents as a condition poorly reported in the literature. Several hypotheses have been proposed to explain the displacement of these elements, however their causes and treatments remain uncertain. The objective of this study is to report a clinical case of inverted impaction of the right lower third molar, where it was chosen exodontia under local anesthesia with conservative osteotomy and total exeresis of the element in a 52-year-old female patient. Third molars with this variation of eruption are rare in the clinic and are usually visualized in routine radiographic examinations and observed clinically after start symptomatology. Several treatments have been proposed, ranging from a surgical approach to long-term follow-up. The intraoral approach associated with conservative management has been shown to be the treatment of choice in most cases. However, the choice of planning will depend, among other factors, on the patient's symptoms and the presence of associated pathologies, always opting for the technique that will promote less operative morbidity and better solubility of the case(AU)

Ionizing radiation and bone quality: time-dependent effects.

BACKGROUND: The aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation. METHODS: Wistar rats were submitted to IR (30 Gy) on the left leg and were euthanized after 30 and 60 days. The legs were divided into four groups according to the treatment and euthanization time: C30 and C60 (right leg-without IR), IR30 and IR60 (left leg-with IR). RESULTS: CT analysis showed more radiodensity in C60 compared with other groups, and IR60 showed more radiodensity than IR30. In histomorphometric analysis, C30 showed lower bone matrix values compared with IR30 and C60. Lacunarity analyses showed more homogeneous bone channel distribution in C30 than IR30. ATR-FTIR showed decrease in ratio of mature and immature crosslinks in IR30 compared with C30. Crystallinity Index was decrease in IR60 compared with C60. The Amide III + Collagen/HA ratio was increased in C60 compared with C30; however this ratio decreased in IR60 compared with IR30. Biomechanical analysis showed lower values in IR groups in both time. CONCLUSIONS: IR damaged bone quality and decreased stiffness. Moreover, the results suggested that the deleterious effects of IR increased in the late time points.

Influence of Platelet-Poor Plasma on Angiogenesis and Maintenance of Volume in Autogenous Bone Grafts.

PURPOSE: The aim of this study was to evaluate the effect of different storage media on angiogenesis and maintaining autogenous bone graft volume in rabbits. MATERIAL AND METHODS: Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was removed and immediately fixed in the right mandibular angle (control group). The other graft was stored for 30 minutes in 1 of the following storage media (n = 6): saline solution (saline group), air exposure (dry group), or platelet-poor plasma (PPP group) and then retained by a screw in the right mandibular angle in the same animal. Four weeks later the animals were euthanized, and the grafted areas were harvested, fixed in 10% phosphate buffered formaldehyde solution, and embedded in paraffin. The 5-µm semi-serial sections were stained in hematoxylin and eosin and Mallory trichrome. RESULTS: Histologic analysis of all groups showed the bone graft was vascularized and well incorporated into the recipient site. The number of blood vessels decreased in the saline and dry groups compared with the control group (P < .03); in contrast, the number of blood vessels increased in the PPP group (P < .05). There were fewer osteoclasts in the saline group compared with the control group (P < .05). Furthermore, the saline group showed larger numbers of blood vessels than the dry group (P < .01). The PPP group showed larger bone graft volumes compared with the dry and saline groups (P < .01). In addition, the saline group showed larger bone graft volumes than the dry group (P < .01). CONCLUSIONS: PPP improved angiogenesis, maintained the volume of the autogenous bone graft, and was a better storage medium during the trans-surgical period than the dry and saline media.

Abordagem clínico-cirúrgica de infecção complexa Em região maxilo-facial: relato de caso / Clinical-surgical approach to complex infection in Maxillofacial region: a case report

Infecções de origem odontogênica possuem o potencial de disseminar-se pelos espaços faciais e comprometer a vida do paciente, devendo, portanto ser tratadas como uma urgência e requerem intervenção imediata. Algumas comorbidades como deficiência imunológica, diabetes e cirrose hepática, podem predispor ao agravamento da infecção e dificultar seu tratamento. Neste relato de caso apresentamos um paciente de 66 anos de idade, com odontalgia em região de molares superiores. Ao exame clínico foi observado sinais flogísticos em fundo de vestíbulo na região de molares e pré-molares superiores direito. Foi iniciada antibioticoterapia por via oral seguida pela extração dos dentes comprometidos. Após 12 horas do primeiro atendimento, o paciente evoluiu com edema extenso de consistência lenhosa em hemiface direita, trismo severo, disfagia, dislalia e dispneia leve. O paciente foi submetido à drenagem cirúrgica do abscesso sob anestesia geral e 72 horas pós-procedimento evoluiu com insuficiência renal aguda, síndrome de abstinência alcoólica, hipertensão arterial sistêmica de difícil controle e piora do quadro infeccioso. Foram solicitados novos exames imaginológicos onde foi observada uma lesão apical inicialmente não diagnosticada associada ao dente 47. O paciente foi submetido à nova drenagem e remoção do dente 47. Após 10 dias do início da nova terapia antibiótica o paciente recebeu alta hospitalar. O mesmo permaneceu em acompanhamento ambulatorial recebendo alta definitiva após 30 dias de acompanhamento em bom estado geral. Para evitar recorrências e instituir um tratamento definitivo para infecções de origem odontogênica, é importante, além da intervenção imediata, um exame minucioso eliminando possíveis focos de infecção

Severe odontogenic infections have the potential to spread through facial spaces and compromise the patient´s life, thus should be treated as an emergency requiring immediate intervention. Some comorbidities such as immunological deficiency, diabetes and liver cirrhousis, can predispose worsening of infection making it difficult to treat. In this case report we present a 66 year old patient with toothache in the region of upper molars. Clinical examination revealed phlogistic signs in the vestibule fundus region of the upper right molars and premolars. Oral antibiotic therapy was initiated followed by extraction of the involved teeth. After 12 hours of the initial treatment, the patient developed extensive edema with woody consistency in right hemiface, severe trismus, dysphagia, dyslalia and slight dyspnoea. The patient underwent surgical drainage of the abscess under general anesthesia. After 72 hours of this procedure he evolved with acute renal failure, alcohol withdrawal syndrome, systemic arterial hypertension of difficult control and worsening of the infectious condition. New imaging exams were requested where an initial undetected apical lesion associated with tooth 47 was observed. The patient was submitted to new drainage and removal of the tooth 47. After 10 days of the start of the new antibiotic therapy the patient was discharged from hospital. The patient remained in outpatient follow-up receiving definitive discharge after 30 days of followup in good general condition. In order to avoid recurrences and to institute a definitive treatment for odontogenic infections, it is important, besides the immediate intervention, a thorough examination eliminating possible foci of infection

High doses of ionizing radiation on bone repair: is there effect outside the irradiated site?

Local ionizing radiation causes damage to bone metabolism, it reduces blood supply and cellularity over time. Recent studies indicate that radiation promotes biological response outside the treatment field. The aim of this study was to investigate the effects of ionizing radiation on bone repair outside the irradiated field. Ten healthy male Wistar rats were used; and five animals were submitted to radiotherapy on the left femur. After 4 weeks, in all animals were created bone defects in the right and left femurs. Seven days after surgery, animals were euthanized. The femurs were removed and randomly divided into 3 groups (n=5): Control (C) (right femur of the non-irradiated animals); Local ionizing radiation (IR) (left femur of the irradiated animals); Contralateral ionizing radiation (CIR) (right femur of the irradiated animals). The femurs were processed and embedded in paraffin; and bone histologic sections were evaluated to quantify the bone neoformation. Histomorphometric analysis showed that there was no significant difference between groups C (24.6±7.04) and CIR (25.3±4.31); and IR group not showed bone neoformation. The results suggest that ionizing radiation affects bone repair, but does not interfere in bone repair distant from the primary irradiated site.

The effects of ionizing radiation on the growth plate in rat tibiae.

The deleterious effects of ionizing radiation on the growth plate continue to be cause for concern. This study evaluated the ionizing radiation effects on bone development and growth plate in the tibia of rats. All animals were submitted to ionizing radiation on the left leg. The animals were divided into two groups and euthanized 30 and 60 days after radiation. The tibiae were removed and separated into groups: control 30 days, irradiated 30 days, control 60 days and irradiated 60 days. Animals in each group (n = 7) were used for macroscopic and histological analysis. The irradiated tibiae showed arrested growth, angular deformity and limb length discrepancy when compared with nonirradiated tibiae. There was statistical difference between control and radiation groups in all the parameters analyzed, except in the lateral-medial thickness of the distal epiphysis. Histological analysis showed evident changes in the growth plate, which was thicker in the Groups irradiated for 30 days, and irradiated for 60 days, compared with their respective controls. The growth plate showed wide areas with disorganized zones of chondrocytes and severely reduced calcification zone. It was concluded that ionizing radiation damaged the growth plate, compromised the endochondral ossification process, and resulted in complete arrest of bone development.

Manipulação gengival para harmonia gengivo-dental em reabilitação unitária anterior com implante û relato de caso / Gingival management for gingival and dental harmony in anterior single rehabilitation with implant û case report

A harmonia gengivo-dental define estética nas reabilitações do arco superior. Em situações, nas quais ocorre perda óssea alveolar no sentido vestíbulo-lingual e ápico-coronal, é necessária associação de técnicas reconstrutivas, visando recuperar volume tecidual. Paciente do gênero masculino, 32 anos, perdeu elemento 11 devido à reabsorção radicular após trauma na região anterior. Foi feita extração dentária, seguida por enxerto ósseo em bloco, instalação de implante e restauração adesiva com dente de estoque. Após o período de osseointegração foi realizado enxerto conjuntivo subepitelial tanto para ganho no sentido vestíbulo-lingual, como ápico-coronal. Imediatamente após o enxerto, iniciou-se o condicionamento gengival com a própria prótese adesiva com pôntico em formato convexo. Em seguida, foi feita a reabertura do implante, instalação de intermediário e confecção de provisório, preservando a arquitetura gengival conseguida. O procedimento provou ser eficiente, garantindo margem gengival e volume de tecido muito próximos do dente adjacente e satisfazendo a expectativa estética do paciente.

The aesthetics of maxillary restorations is defined by the harmony between gingiva and teeth. gingival-dental harmony defines the aesthetics in the upper arch rehabilitation. In cases of alveolar bone loss in buccolingual and apical-coronal direction an association of techniques is required to recover the tissues volume. Male patient, 32 years old, lost element 11 due to radicular resorption, after trauma in the anterior region. Tooth extraction, block bone graft, implant installation and adhesive restoration with a stock tooth were performed. After osseointegration period, a subepithelial connective tissue graft was made to have gain in both directions, buccolingual and apical-coronal. Immediately after the graft, gingival conditioning started with the same adhesive prosthesis with convex shape pint. Then implant was reopened, abutment was installed and the preparation of provisional was executed, preserving the achieved gingival architecture. The procedure proved to be efficient, ensuring gingival margin and tissue volume very close to the adjacent tooth and satisfying the aesthetic expectations of the patient.

The Effect of Local Delivery Doxycycline and Alendronate on Bone Repair.

The aim of the present study was to investigate the local effect of 10% doxycycline and 1% alendronate combined with poly(lactic-co-glycolic acid) (PLGA) on bone repair. Thirty rats were divided into three groups, as follows: control group (CG), drug group (DG), and vehicle-PLGA group (VG). Bone defect was created in the right femur and filled with the following: blood clot (CG); PLGA gel, 10% doxycycline and 1% alendronate (DG); or vehicle-PLGA (VG). The animals were euthanized 7 or 15 days after surgery. Bone density, bone matrix and number of osteoclasts were quantified. At 7 days, the findings showed increased density in DG (177.75 ± 76.5) compared with CG (80.37 ± 27.4), but no difference compared with VG (147.1 ± 41.5); no statistical difference in bone neoformation CG (25.6 ± 4.8), VG (27.8 ± 4), and DG (18.9 ± 7.8); and decrease osteoclasts in DG (4.6 ± 1.9) compared with CG (26.7 ± 7.4) and VG (17.3 ± 2.7). At 15 days, DG (405.1 ± 63.1) presented higher density than CG (213.2 ± 60.9) and VG (283.4 ± 85.8); there was a significant increase in percentage of bone neoformation in DG (31.5 ± 4.2) compared with CG (23 ± 4), but no difference compared with VG (25.1 ± 2.9). There was a decreased number of osteoclasts in DG (20.7 ± 4.7) and VG (29.5 ± 5.4) compared with CG (40 ± 9.4). The results suggest that the association of 10% doxycycline and 1% alendronate with PLGA-accelerated bone repair.

Low-level laser therapy on bone repair: is there any effect outside the irradiated field?

The biological effects of local therapy with laser on bone repair have been well demonstrated; however, this possible effect on bone repair outside the irradiated field has not been evaluated. The aim of this study was to investigate the effect of low-level laser therapy (LLLT) (λ = 830 nm) on repair of surgical bone defects outside the irradiated field, in rats. Sixty Wistar rats were submitted to osteotomy on the left femur and randomly separated into four groups (n = 15): group I, control, bone defect only; group II, laser applied on the right femur (distant dose); group III, laser applied locally on the bone defect and also on the right femur (local and distant doses); and group IV, laser applied locally on the left femur (local dose). Laser groups received applications within a 48-h interval in one point per session of density energy (DE) = 210 J/cm(2), P = 50 mW, t = 120 s, and beam diameter of 0.028 cm. Five animals of each group were euthanized 7, 15, and 21 days after surgery. Histologic analysis in all groups showed new bone formation in the region of interest (ROI) at 7 days. After 15 days, bone remodeling with a decrease of bone neoformation in the marrow area was observed in all groups. After 21 days, advanced bone remodeling with new bone mostly located in the cortical area was observed. The histomorphometric analysis showed at 7 days a significant increase of bone formation in groups III and IV compared to groups I and II. At days 15 and 21, histomorphometric analysis showed no significant differences between them. Laser therapy presented a positive local biostimulative effect in the early stage of bone healing, but the LLLT effect was not observed a long distance from the evaluated area.

Tratamento cirúrgico de fratura mandibular bilateral em paciente pediátrico / Surgical treatment of bilateral mandibular fracture in a pediatric patient

Fraturas mandibulares na infância são raras e seu tratamento permanece controverso. Fatores como dentição decídua, mista ou permanente e estágio de desenvolvimento ósseo devem ser considerados para escolha do tratamento, que varia desde restrição de dieta, fixação intermaxilar, odontossíntese e até fixação interna com dispositivos de titânio ou reabsorvíveis. Esse relato descreve o tratamento cirúrgico, realizado por acesso intrabucal, de fratura bilateral de mandíbula, em uma paciente de 9 anos de idade. As fraturas foram fixadas com uma miniplaca de titânio na base mandibular e odontossíntese; e, após um ano, as placas foram removidas. A paciente apresenta boa oclusão dentária e nenhum prejuízo funcional ou estético... (AU)

During childhood, mandibular fractures are rare, and their treatment remains controversial. Factors such as primary, mixed or permanent dentition and stage of bone development should be considered when deciding on the choice of the treatment, which ranges from diet restriction, intermaxillary fixation and circumdental wiring to internal fixation with titanium or resorbable devices. This case report describes the surgical treatment, using the intraoral approach, of a bilateral mandibular fracture, in a nine-year-old patient. The fractures were fixed with a titanium miniplate on the mandibular base and circumdental wiring, the plate being removed after one year. The patient shows a good dental occlusion and no aesthetic or functional injury... (AU)

Effect of different storage media on the regenerative potential of autogenous bone grafts: a histomorphometrical analysis in rabbits.

The success of autogenous bone graft is related to the graft cell viability. In bone-grafting procedures, harvested grafts are often maintained in extraoral media while the recipient site is prepared. The aim of this study was to evaluate in vivo the effect of storage media over autogenous bone grafts during the transsurgical time. Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was immediately fixed in the mandibular angle (control group), and the other was maintained in air exposure (dry group), 0.9% NaCl solution (saline group), or platelet-poor plasma (PPP group) during 30 minutes and stabilized in the symmetrical location of control grafts. After 28 days, the animals were euthanized and the bone fragments were removed, demineralized, and embedded in paraffin. Histological evaluation was performed under light microscope. Empty lacunae and bone graft area quantification were carried out for the sections. The histomorphometrical analysis revealed reduction of the graft area and increase of empty lacunae in the dry group when compared with control. No significant differences were found in the number of empty lacunae or bone graft area between the saline group and its control and also between the PPP group and its control. The dry group showed more empty lacunae and less graft area than the saline and PPP groups. In accordance with the results, PPP and physiologic solution demonstrated osteocyte preservation and bone graft area maintenance, being satisfactory storage media for autogenous bone grafts during the transsurgical period.

Enxerto bovino orgânico associado ao PRP em calvária de coelhos / Organic bovine graft associated with PRP in rabbit calvaria

Introdução: O reparo ósseo de grandes defeitos é um grande desafio para a cirurgia reconstrutora atualmente. Objetivo: O objetivo desse estudo foi realizar avaliação histológica do reparo ósseo em calvária de coelhos depois do uso de enxerto ósseo bovino (Gen-ox-organic®) associado a plasma rico em plaquetas (PRP). Método: Foram utilizados 12 coelhos, e dois fragmentos ósseos foram removidos da calvária bilateralmente. Então 24 sítios cirúrgicos foram aleatoriamente separados em 3 grupos: coágulo (grupo I), orgânico (grupo II) e orgânico com PRP (grupo III). Depois de quatro semanas, os animais foram sacrificados e a área enxertada foi removida, fixada em formol a 10%, em PBS 0,1M e incluídas em parafina. Resultados: Os parâmetros histológicos analisados foram: área do defeito preenchida com osso neoformado, presença de células gigantes e partículas do enxerto, e neoformação óssea associada com as partículas. Os defeitos do grupo I foram preenchidos com tecido fibroso que condicionou o periósteo e apresentou uma pequena formação óssea na periferia. Nos grupos II e III, um padrão semelhante foi observado e também ausência de partículas do enxerto e células gigantes. Não houve diferença significativa no número de células gigantes, partículas do enxerto e neoformação óssea em volta das partículas entre o material enxertado e o grupo com PRP associado. Conclusão: Os resultados obtidos indicam que o biomaterial orgânico isolado ou em associação com o PRP não melhoraram a regeneração óssea.

Introduction: Repairing large bone defects is a huge challenge that reconstructive surgery currently faces. Objective: The objective of this study was to perform the histological evaluation of bone repair in rabbit calvaria when using bovine bone graft (Gen-ox-organic®) associated with platelet-rich plasma (PRP). Method: 12 rabbits were used and two bone fragments were bilaterally removed from calvaria. Then, 24 surgical sites were randomly divided into 3 groups: coagulum (group I), organic (group II) and PRP-included organic (group III). After four weeks, the animals were sacrificed and the grafted area removed, fixed in 10% formalin with PBS 0.1 M, and embedded in paraffin. Study method: The analyzed histological parameters were: defective area filled with the newly-formed bone, graft's giant cells and particles, as well as the new bone formation associated with the particles. Group I's defects were filled with fibrous tissue attaching the periosteum and revealed a little bone formation peripherally. In both groups II and III, a similar standard was noticed in addition to the absence of graft particles and giant cells. There was no significant difference in the number of giant cells, graft particles and new bone formation around the particles between the grafted material and the PRP-related group. Conclusion: The results achieved indicate that the organic biomaterial neither separately nor jointly with PRP improves bone regeneration.